Thermo Fisher Scientific HSC70 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 470 and 635 of Human Hsc70 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSC70,uniProtId:P11142-1,ncbiNodeId:9606,antigenRange:470-635,antigenLength:646,antigenImageFileName:PA5-27337_HSC70_P11142-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27337_HSC70_P11142-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544813

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.

Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (91%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.