Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT383)
HMGB1 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 액상 형태, 보존제 무첨가. 염증 및 세포 신호전달 연구에 적합.
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Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT383)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT383 |
| Immunogen | Recombinant fragment (aa 2–159 of HMGB1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; -20°C long term, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538743 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, mouse brain, rat brain
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 extracellularly, where it functions as a proinflammatory cytokine. Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains: two positively charged DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162) and a negatively charged 30 aa C-terminal acidic tail. Nuclear localization signals are found at residues 28–44 and 180–185. The B box mediates cytokine activity, while the A box interacts with transcription factors. HMGB1 promotes monocyte migration, cytokine secretion, and T cell–dendritic cell interaction via receptors RAGE, TLR2, and TLR4. It is highly conserved, present in nuclei and cytoplasm of most cell types, and acts as a key mediator of inflammation in sterile and infection-associated responses. Elevated HMGB1 levels are observed in tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.
Usage
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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