Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT383)

Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT383)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, mouse brain, rat brain
• Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 extracellularly, where it functions as a proinflammatory cytokine. Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains: two positively charged DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162) and a negatively charged 30 aa C-terminal acidic tail. Nuclear localization signals are found at residues 28–44 and 180–185. The B box mediates cytokine activity, while the A box interacts with transcription factors. HMGB1 promotes monocyte migration, cytokine secretion, and T cell–dendritic cell interaction via receptors RAGE, TLR2, and TLR4. It is highly conserved, present in nuclei and cytoplasm of most cell types, and acts as a key mediator of inflammation in sterile and infection-associated responses. Elevated HMGB1 levels are observed in tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.

Usage

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.