Thermo Fisher Scientific NFkB p65 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

View 17 publications 17 publications

Immunohistochemistry (IHC)

-

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

View 11 publications 11 publications

CUT&RUN (C&R)

-

- -

Product Specifications

Species Reactivity

Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Pig, Rabbit, Rat, Xenopus

Published species

Bovine, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the C-terminus of human NFkappaB protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFkB p65,uniProtId:Q04206-1,ncbiNodeId:9606,antigenRange:551,antigenLength:551,antigenImageFileName:PA5-16545_NFkB_p65_Q04206-1_Rabbit.svg,antigenImageFileNamePDP:PA5-16545_NFkB_p65_Q04206-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice (domestic); Dry ice (international)

RRID

AB_10981081

Product Specific Information

PA5-16545 targets NFkappaB/p65 in IHC (P), IF, and WB applications and shows reactivity with mouse, Rabbit, Rat, Xn, Bovine, Canine, Drosophila, Hamster, Human, and Porcine samples.

The PA5-16545 immunogen is a synthetic peptide from the C-terminus of human NFkappaB protein.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.