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Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), APC-eFluor 780, eBioscience

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인간 ICOS(CD278)를 인식하는 ISA-3 단클론 항체로, APC-eFluor 780 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 활성화된 T세포의 공동자극 분석에 적합합니다. 4°C 암소 보관, 동결 금지.

카탈로그번호: 47-9948-4x (2개 옵션)
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 47-9948-42 CD278 (ICOS) Monoclonal Antibody (ISA-3), APC-eFluor 780, eBioscience 100 tests pk판매 단위 pk ·

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Thermo Fisher Scientific 47-9948-41 CD278 (ICOS) Monoclonal Antibody (ISA-3), APC-eFluor 780, eBioscience 25 tests pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), APC-eFluor 780, eBioscience

Applications and Tested Dilution

Application	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 11 publications
Functional Assay	-	View 1 publication

Product Specifications

항목	내용
Published species	Human
Host / Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	ISA-3
Conjugate	APC-eFluor™ 780
Excitation/Emission Max	756/785 nm
Form	Liquid
Concentration	5 µL/Test
Storage conditions	4°C, store in dark, DO NOT FREEZE
Shipping conditions	Wet ice
RRID	AB_2573999

Available Formats

Unconjugated
Alexa Fluor 700
APC
Biotin
Brilliant UV 661
eFluor 450
FITC
Functional Grade
NovaFluor Red 660
NovaFluor Red 710
NovaFluor Yellow 700
NovaFluor Yellow 730
PE
PE-Cyanine5.5
PE-Cyanine7
PE-eFluor 610
PerCP-eFluor 710
Super Bright 436
Super Bright 600
Super Bright 645
Super Bright 780
Request custom conjugation

Product Specific Information

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM.
ICOS is a T-cell-specific activation molecule and a member of the CD28/CTLA-4 family. It plays a critical role in T-cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation.

ICOS binds to its ligand B7h/B7RP-1 expressed on activated APCs and inflamed tissues.
Plate-bound ISA-3 induces IL-4, IL-5, IL-10 production but not IL-2.
ISA-3 was generated against human ICOS antigen.
Clone C398.4A (anti-mouse ICOS/H4) cross-reacts with human ICOS but binds a different epitope.
For brightest staining of activated human T cells, use Product #13-9948-82 or #12-9948-42.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg)/test.
Laser Excitation: Red laser (633 nm)
Emission: 780 nm

Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.

Filtration: 0.2 µm post-manufacturing filtered.