Thermo Fisher Scientific CD303a Monoclonal Antibody (201A), APC-eFluor 780, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

201A

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

View additional formats

• APC
• eFluor 450
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784717

Product Specific Information

Description: This 201A monoclonal antibody reacts with human CD303a, which is also known as CLEC4C or Blood Dendritic Cell Antigen 2 (BDCA-2). This type II transmembrane protein is expressed almost exclusively on plasmacytoid dendritic cells, which secrete type I interferon during antiviral and antitumor responses. Studies have demonstrated that CD303a binds asialo-galactosyl-oligosaccharides on invading pathogens, resulting in the activation of signaling pathways that lead to CD303a internalization and downregulation of interferon production.

Applications Reported: This 201A antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 201A antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

This gene encodes a member of the C-type lectin/C-type lectin-like domain superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in dendritic cell function. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.