Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), PE, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 0.25 µg/test

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Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation.

CD106 binds to integrin alpha4 beta1 (VLA-4, CD49d/CD29) and Integrin α4β7 (LPAM-1). These interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤ 0.25 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, adhesion, and signal transduction, and may play a role in inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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