Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience
인간 CD107a(LAMP-1)을 인식하는 eBioH4A3 단클론 항체로, PE 형광 표지된 제품입니다. 세포 내 염색 및 Flow Cytometry 분석에 최적화되어 있으며, 친화 크로마토그래피로 정제되었습니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 12-1079-4x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience
Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 20 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH4A3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852862 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- eFluor 660
- FITC
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated ~110 kDa protein expressed mainly in lysosomal/endosomal membranes of nearly all cells. It transiently appears on the cell surface of degranulating cytolytic T cells and is upregulated in activated platelets and certain cancer cells.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Surface staining in flow cytometric degranulation assays
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test.
Excitation / Emission / Laser:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible with Blue, Green, Yellow-Green lasers
Filtration:
0.2 µm post-manufacturing filtered
Target Information
LAMP1 (CD107a) is a major component of the lysosomal membrane, typically co-expressed with LAMP2. About 1–2% of CD107a is found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes.
Upon activation, LAMP1 translocates to the plasma membrane via a carboxyl-terminal tyrosine-based motif (YXXI). Mutations affecting this motif alter lysosomal localization, causing cycling between plasma membrane and endosome. Cell surface LAMP1 and LAMP2 promote PBMC adhesion to vascular endothelium, suggesting roles in inflammation. Elevated LAMP1 expression is observed around senile plaques in Alzheimer’s disease and in various immune cells during thymic involution. LAMP1 serves as an effective marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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