Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience

Thermo Fisher Scientific CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated ~110 kDa protein expressed mainly in lysosomal/endosomal membranes of nearly all cells. It transiently appears on the cell surface of degranulating cytolytic T cells and is upregulated in activated platelets and certain cancer cells.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis
• Surface staining in flow cytometric degranulation assays

Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible with Blue, Green, Yellow-Green lasers

Filtration:
0.2 µm post-manufacturing filtered

Target Information

LAMP1 (CD107a) is a major component of the lysosomal membrane, typically co-expressed with LAMP2. About 1–2% of CD107a is found on the plasma membrane. It is a heavily glycosylated type I integral membrane protein involved in lysosome biogenesis and fusion with phagosomes.

Upon activation, LAMP1 translocates to the plasma membrane via a carboxyl-terminal tyrosine-based motif (YXXI). Mutations affecting this motif alter lysosomal localization, causing cycling between plasma membrane and endosome. Cell surface LAMP1 and LAMP2 promote PBMC adhesion to vascular endothelium, suggesting roles in inflammation. Elevated LAMP1 expression is observed around senile plaques in Alzheimer’s disease and in various immune cells during thymic involution. LAMP1 serves as an effective marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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