Thermo Fisher Scientific Spectrin beta-3 Polyclonal Antibody
Spectrin beta-3 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, IP 등에 사용 가능. Human 및 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 세포골격 연구 및 신경퇴행성 질환 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10–20 µg/mL | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The epitope recognized by this antibody maps to a C-terminal region between residue 2340 and 2390 of spectrin beta 3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2194518 |
Product Specific Information
- Suggested positive control: HeLa whole cell extract.
Target Information
Spectrins are principal components of the cell membrane-cytoskeleton, composed of two alpha and two beta spectrin subunits.
The SPTBN2 gene encodes spectrin beta non-erythrocytic 2 (beta-III spectrin), which stabilizes the glutamate transporter EAAT4 at the plasma membrane surface.
Mutations in SPTBN2 cause spinocerebellar ataxia type 5 (SCA5), a neurodegenerative disorder characterized by progressive locomotor incoordination, dysarthria, and uncoordinated eye movements.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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