Thermo Fisher Scientific Phospho-CaMKII alpha/beta/delta (Thr305) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CAMK2A(Accession Q9UQM7), corresponding to amino acid residues around phosphorylated Thr305. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaMKII alpha/beta/delta,uniProtId:Q9UQM7-1,ncbiNodeId:9606,antigenRange:305,antigenLength:478,antigenImageFileName:PA5-105042_CaMKII_alphabetadelta_Q9UQM7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105042_CaMKII_alphabetadelta_Q9UQM7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816515

Product Specific Information

Antibody detects endogenous levels of CaMK2 alpha/beta/delta only when phosphorylated at Threonine 305.

Target Information

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be co-expressed in the same tissue type. CaMKII alpha is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.