Thermo Fisher Scientific CD39 Monoclonal Antibody (5F2), PE, eBioscience

Thermo Fisher Scientific CD39 Monoclonal Antibody (5F2), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 2 publications

Product Specifications

Product Specific Information

This 5F2 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI.
NTPDases convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), removing toxic extracellular ATP and ADP.
CD39, the predominant member in the immune system, plays a role in inflammation suppression and platelet activation control.
It influences CD73 expression, together modulating inflammatory responses.

CD39 is expressed on:

• B cells
• Langerhans cells
• Most monocytes
• A subset of CD4+ T cells (mostly CD25+FoxP3+ T regs)

T reg cells from CD39⁻ mice show impaired suppressive properties in vitro and in vivo.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes
Use ≤ 0.5 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD39 cell surface antigen (70–100 kD) is expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes.
It possesses intrinsic ecto-ATPase activity, induced upon T cell activation and increased on B cells as a late activation antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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