Thermo Fisher Scientific CD325 (N-Cadherin) Monoclonal Antibody (8C11), PE, eBioscience

Thermo Fisher Scientific CD325 (N-Cadherin) Monoclonal Antibody (8C11), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, consisting of five extracellular repeats, a transmembrane domain, and a cytoplasmic domain. CD325-deficient mice die at day 10 of gestation and display major heart defects and malformed neural tubes and somites. CD325 is involved in cardiac development, including precardiac mesoderm formation, left-right symmetry establishment, and cardiac looping morphogenesis. It also induces cell cycle arrest, and its expression is often dysregulated in cancer cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on HeLa cells at 5 µL (0.25 µg) per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

N-cadherin is a 140 kDa transmembrane protein mediating calcium-dependent intercellular adhesion. It regulates morphogenetic movements during development and controls cell surface adhesion through homotypic interactions. Its function depends on association with the actin-cytoskeleton via catenin proteins, regulated by phosphorylation of beta-catenin. N-cadherin comprises five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail, functioning during gastrulation and required for left-right asymmetry establishment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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