Thermo Fisher Scientific Synaptotagmin 1 Polyclonal Antibody
Rabbit polyclonal antibody against human Synaptotagmin 1 for WB and IHC applications. Recognizes recombinant protein corresponding to amino acids 97–165. Affinity purified, liquid form, unconjugated, supplied in PBS with glycerol. Ideal for studying Ca...
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Synaptotagmin 1 (aa 97–165). Recombinant protein control fragment: Product #RP-88816 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2856158 |
Product Specific Information
Immunogen sequence:
GGKNAINMKD VKDLGKTMKD QALKDDDAET GLTDGEEKEE PKEEEKLGKL QYSLDYDFQN NQLLVGIIQ
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein widely expressed throughout the CNS and functions as a Ca²⁺ sensor in exocytosis and neurotransmitter release. Its Ca²⁺-mediated binding of SNAP25 is essential for Ca²⁺-dependent membrane triggering. Specific residues within the C2B domain determine endocytic rate and vesicle size. Synaptotagmin 1 regulates synaptic vesicle trafficking and binds acidic phospholipids requiring both acidic head groups and diacyl backbones. It interacts with protein kinase C receptors in a Ca²⁺-dependent manner and binds neurexins, syntaxin, and AP2 independently of Ca²⁺.
Diseases associated with Synaptotagmin 1 dysfunction include food-borne and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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