Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB.
Common laboratory strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For these strains, use the DX5 monoclonal antibody (Product # 14-5971).

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers by a majority of cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤1 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.

Fluorochrome Information:

• Alexa Fluor® 700 emits at 723 nm
• Excitation: 633–647 nm
• Laser: Red
• Recommended filters: 685 LP mirror, 710/20 filter
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161) is a C-type lectin-like receptor expressed by natural killer (NK) cells.
It mediates cytotoxicity and cytokine secretion upon immune stimulation. The KLRB1 protein features extracellular lectin motifs, a transmembrane domain, and a cytoplasmic domain, classifying it as a type II membrane protein.
NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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