Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Alexa Fluor 700, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, Alexa Fluor 700 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며 696/719 nm의 발광 특성을 가집니다. C57BL 및 NZB 계열 마우스 NK 세포 검출에 적합합니다.
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Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 46 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574504 |
Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB.
Common laboratory strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For these strains, use the DX5 monoclonal antibody (Product # 14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers by a majority of cells, with small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤1 µg per test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.
Fluorochrome Information:
- Alexa Fluor® 700 emits at 723 nm
- Excitation: 633–647 nm
- Laser: Red
- Recommended filters: 685 LP mirror, 710/20 filter
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161) is a C-type lectin-like receptor expressed by natural killer (NK) cells.
It mediates cytotoxicity and cytokine secretion upon immune stimulation. The KLRB1 protein features extracellular lectin motifs, a transmembrane domain, and a cytoplasmic domain, classifying it as a type II membrane protein.
NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as its functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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