Thermo Fisher Scientific PP2A alpha Polyclonal Antibody
PP2A alpha 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성이 있으며, 고순도 Affinity Chromatography로 정제된 액상 제품입니다. 연구용으로 세포 내 위치 분석에 활용됩니다.
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Thermo Fisher Scientific PP2A alpha Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–500 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–309 of human PP2A Catalytic alpha (NP_0027061) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 8.49 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806646 |
Product Specific Information
Immunogen sequence:
MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFLPositive Samples: SW480, BT-474, 293T, MCF7, Mouse brain, Mouse spleen, Rat lung, Rat brain
Cellular Location: Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Spindle pole
Target Information
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues regulate essential cellular functions such as division, homeostasis, and apoptosis. Protein phosphatases are key components in this process. The protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit, and a catalytic subunit. Four major families of catalytic subunits are identified: PP1, PP2A, PP2B (calcineurin), and PP2C. The PP2A family includes PP2A alpha and PP2A beta subfamily members, while PPX (PP4) represents a putative novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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