Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Thermo Fisher의 Rabbit anti-Mouse IgG (H+L) AP 결합 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 교차흡착 처리로 비특이적 반응을 최소화하며, 높은 신호 증폭과 다양한 검출 시스템에 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534840 |
| Target | IgG |
| Cross Adsorption | Against human serum proteins |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
다양한 포맷으로 제공됩니다:
- Alexa Fluor 350, 488, 555, 568, 594, 633, 647, 680
- Biotin
- DyLight 488, 550, 650, 680, 755, 800
- FITC
- HRP
- TRITC
- 맞춤 결합(Custom Conjugation) 요청 가능
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. These antibodies are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility by enabling use with multiple detection systems (e.g., HRP, AP, fluorescence) and improve sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, or label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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