Thermo Fisher Scientific POLB Polyclonal Antibody
Rabbit polyclonal antibody targeting human POLB, suitable for Western blot. Recognizes conserved peptide sequence across multiple species. Supplied as liquid, unconjugated form with 0.5 mg/mL concentration. For research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 0.2–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the middle region of human POLB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2609682 |
Product Specific Information
Peptide sequence:
GPSAARKFVD EGIKTLEDLR KNEDKLNHHQ RIGLKYFGDF EKRIPREEMLSequence homology:
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Target Information
DNA polymerase beta comprises an amino-terminal 8 kDa domain and a carboxy-terminal 31 kDa domain.
The N-terminal ssDNA binding domain has deoxyribose phosphodiesterase activity, while the C-terminal domain has nucleotidyltransferase activity.
Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells. Treatment of cells with DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level.
DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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