Thermo Fisher Scientific UNG Monoclonal Antibody (OTI1A11), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These isoforms are generated by alternative splicing and use of two differentially regulated promoters (PA and PB).
UNG1 and UNG2 cDNAs are similar in size (2061 and 2058 bp), and their mRNAs are not resolved as distinct species in gel electrophoresis.
UNG2 differs from UNG1 by 44 amino acids at the N-terminal region, which is not essential for catalytic activity.

The UNG gene products play a major role in repairing mutagenic U:G mispairs caused by cytosine deamination. For instance, UNG2 removes misincorporated dUMP residues. Expression levels of UNG1 and UNG2 vary by cell and tissue type and are regulated throughout the cell cycle. Generally, UNG expression and activity are higher in proliferating cells compared to nonproliferating ones.
UNG1 is a 304 amino acid protein, while UNG2 is a 313 amino acid protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.