Thermo Fisher Scientific HCN2 Monoclonal Antibody (S71), PerCP
HCN2 단백질을 인식하는 마우스 단클론 항체로 PerCP로 결합됨. Western blot, IHC, ICC 등 다양한 응용에 적합. 인간, 생쥐, 랫트 반응성. 단백질 G 정제, 보존제 없음. 연구용으로만 사용 가능.
- 카탈로그번호
- MA545405
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S71 |
| Immunogen | Fusion protein amino acids 761–863 (cytoplasmic C-terminus) of rat HCN2 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931859 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45405 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 95 kDa. No cross-reactivity against HCN1.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play important roles in motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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