Thermo Fisher Scientific IL-6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

ELISA (ELISA)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human IL6, expressed in E.coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

80 mg/mL

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_794649

Product Specific Information

This antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.