Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

This L161 monoclonal antibody detects CD1c (BDCA-1), a glycoprotein noncovalently linked to beta-2 microglobulin on thymocytes and antigen-presenting cells such as dendritic and Langerhans cells. It is also expressed on certain circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in presenting lipid antigens (e.g., microbial fatty acids) to effector T cells during the adaptive immune response. Alternative splicing yields three isoforms: soluble, membrane, and cytoplasmic/soluble.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use at 5 µL (0.06 µg) per test in a final volume of 100 µL.

Note:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample before combining with cells. For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton™ dye structures engineered for optimized fluorescence signatures and minimal spectral spillover.
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue)

Target Information

The CD1 family comprises transmembrane glycoproteins structurally related to MHC proteins, forming heterodimers with beta-2-microglobulin. These proteins present lipid and glycolipid antigens to T cells. Human CD1 genes are clustered on chromosome 1 and differ in localization and ligand specificity. CD1c localizes to endosomes and lysosomes via a tyrosine-based motif and requires vesicular acidification for lipid antigen binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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