Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.1 µg)/test
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometric analysis of normal human peripheral blood cells. Recommended use: 5 µL (0.06 µg) per test.

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

This L161 monoclonal antibody detects CD1c (BDCA-1), a glycoprotein linked to beta-2 microglobulin on thymocytes and antigen-presenting cells such as dendritic and Langerhans cells. CD1c is also expressed on certain B cells, lymph nodes, and germinal centers. It presents lipid antigens to effector T cells during adaptive immune responses.
Alternative splicing gives rise to soluble, membrane, and cytoplasmic/soluble isoforms.

Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

NovaFluor dye compatibility:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best results.

CellBlox Plus Blocking Buffer:
Updated formulation (Cat. No. C001T06F01) required for optimal staining.
Add 5 µL per sample to antibody cocktails or single-color controls (100 µL cell sample, 10³–10⁸ cells).

NovaFluor conjugates:
Based on Phiton™ technology, engineered for minimal spillover and spectral spread.
Excitation: 639 nm; Emission: 710 nm; Laser: 633–640 nm (Red).

Target Information

CD1 family proteins are transmembrane glycoproteins related to MHC molecules, forming heterodimers with beta-2-microglobulin. They present lipid and glycolipid antigens to T cells.
Human genome contains five CD1 genes on chromosome 1.
This protein localizes to late endosomes and lysosomes, requiring vesicular acidification for lipid antigen binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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