Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific CD1c Monoclonal Antibody (L161), NovaFluor Blue 690

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• APC
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available upon request

Product Specific Information

This L161 monoclonal antibody detects CD1c (BDCA-1), a glycoprotein noncovalently linked to beta-2 microglobulin on thymocytes and antigen-presenting cells such as dendritic and Langerhans cells. It is also expressed on certain circulating and marginal zone B cells, lymph nodes, and germinal centers.

CD1c is involved in presenting lipid antigens (e.g., microbial fatty acids) to effector T cells during adaptive immune responses. Alternative splicing produces three isoforms: soluble, membrane, and cytoplasmic/soluble.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on normal human peripheral blood cells. Recommended use: 5 µL (0.2 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer:

• Cat. No. C001T06F01
• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample (regardless of number of NovaFluors)
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells)

NovaFluor Technology:
Based on Phiton™ technology using novel nucleic acid dye structures engineered for minimal spillover and spread.
Excitation: 494 nm; Emission: 585 nm; Laser: 488 nm (Blue)

Target Information

The CD1 family encodes transmembrane glycoproteins related to MHC proteins, forming heterodimers with beta-2-microglobulin. These mediate presentation of lipid and glycolipid antigens to T cells. Five CD1 genes are clustered on chromosome 1, differing in localization and ligand specificity. CD1c localizes to late endosomes and lysosomes via a tyrosine-based motif, requiring vesicular acidification for lipid antigen binding.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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