Thermo Fisher Scientific IDO Polyclonal Antibody
인간 시료의 Indoleamine 2,3-dioxygenase(IDO)를 검출하는 Thermo Fisher Scientific의 sheep polyclonal 항체입니다. ELISA, Flow Cytometry, IHC, Western blot 등 다양한 응용에 사용 가능하며, 1:50 희석 비율로 권장됩니다. 단백질 A 정제, PBS/BSA buffer, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant IDO |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2295818 |
Product Specific Information
PA1-40279 detects Indoleamine 2,3-dioxygenase (IDO) from human samples.
It has been successfully used in ELISA, flow cytometry, immunohistochemistry (paraffin), Western blot, and immunofluorescence applications.
A typical starting dilution is 1:50. For flow cytometry, it is recommended to use the antibody with fixing and permeabilization.
The PA1-40279 immunogen is recombinant IDO.
Target Information
IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of regulatory molecules such as tryptophan, serotonin, and melatonin.
This reaction initiates the production of biologically active metabolites known as kynurenines.
IDO1 is widely expressed in various human tissues, macrophages, and dendritic cells.
During inflammation, interferons (IFNs) trigger IDO1 induction, contributing to antimicrobial mechanisms by degrading tryptophan and inhibiting pathogen growth.
IDO1 also plays a crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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