Thermo Fisher Scientific Complement C4d Polyclonal Antibody
인체 C4d 단백질을 인식하는 Thermo Fisher의 폴리클로날 항체로, IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼에 1% BSA와 나트륨 아지드가 포함되어 있습니다. 연구용으로만 사용되며 -20°C에서 보관합니다.
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Applications
Tested Dilution
- Immunohistochemistry (Paraffin) (IHC (P)): 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human C4d |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979257 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b.
Together C2a and C4b form the classical C3 convertase.
The molecular mass of C4 is 204 kDa and it consists of three subunits: α (97 kDa), β (75 kDa), and γ (33 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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