Thermo Fisher Scientific Complement C4d Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human C4d if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Complement C4d,uniProtId:P0C0L4-1,ncbiNodeId:9606,antigenRange:1744,antigenLength:1744,antigenImageFileName:PA1-39488_Complement_C4d_P0C0L4-1_Rabbit.svg,antigenImageFileNamePDP:PA1-39488_Complement_C4d_P0C0L4-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Complement C4d,uniProtId:P0C0L5-1,ncbiNodeId:9606,antigenRange:1744,antigenLength:1744,antigenImageFileName:PA1-39488_Complement_C4d_P0C0L5-1_Rabbit.svg,antigenImageFileNamePDP:PA1-39488_Complement_C4d_P0C0L5-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979257

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

Target Information

C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase. The molecular mass of C4 is 204 kDa and it consists of 3 subunits: a (97 kDa), b (75 kDa) and g (33 kDa).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.