Thermo Fisher Scientific Phospho-S6 (Ser240, Ser244) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
• After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
• Suggested positive control: breast carcinoma.
• Originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. When used separately, performance may vary and optimization might be required.

Target Information

Ribosomes are organelles responsible for protein synthesis, composed of a small 40S subunit and a large 60S subunit, which together contain 4 RNA species and about 80 distinct proteins.
The encoded protein is a cytoplasmic ribosomal protein and a component of the 40S subunit, belonging to the S6E family. It is a major substrate for multiple protein kinases, which phosphorylate five C-terminal serine residues in response to various stimuli such as growth factors, tumor promoters, and mitogens.
Dephosphorylation occurs during growth arrest. This protein may play a role in regulating cell growth and proliferation by selectively translating specific classes of mRNA.
Multiple processed pseudogenes of this gene are dispersed throughout the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.