Thermo Fisher Scientific beta Tubulin Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• This product is preservative-free.
• It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
• This antibody has specificity for Human beta Tubulin (TUBB).

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene can cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin has also been detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, inhibiting tubulin incorporation into microtubules.

Microtubules, major cytoskeletal elements found in all eukaryotic cells, consist of tubulin dimers (alpha and beta subunits, ~55 kDa each). They play essential roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support. Beta-tubulin is ubiquitously expressed in all eukaryotic cells and is commonly used as a loading control in protein detection assays such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.