Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), PE, eBioscience
Mouse CD140b (PDGFRB) 단백질을 인식하는 PE-conjugated 단클론 항체. Flow cytometry 및 세포 분석용으로 적합하며, 488–561 nm에서 여기, 578 nm에서 방출. 4°C 암조건 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 1 µg/test | 36 |
| In vitro Assay (IV) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | APB5 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_529484 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Custom conjugation available
Product Specific Information
The APB5 monoclonal antibody reacts with mouse CD140b (PDGFRB), the beta chain of the platelet-derived growth factor receptor. PDGFRB is a receptor tyrosine kinase that forms dimers (α/α, β/β, or α/β) upon ligand binding and phosphorylates substrates. It is expressed in embryonic and mesenchymal-derived adult mouse tissues and plays a role in fibrous atherosclerotic lesion formation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on NIH/3T3 cells at ≤1 µg/test (100 µL final volume). Optimal titration recommended.
Optical Properties: Excitation 488–561 nm; Emission 578 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PDGFRB is a receptor tyrosine kinase involved in atherogenesis and SIS oncogene-mediated transformation. It acts as a mitogen for mesenchymal-origin cells. Depending on the ligand, PDGFRB forms homodimers or heterodimers with PDGFRA. The gene is located on chromosome 5, near GM-CSF and M-CSFR genes, all implicated in 5q syndrome. A translocation between chromosomes 5 and 12 fusing PDGFRB with ETV6 is associated with chronic myeloproliferative disorders with eosinophilia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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