Thermo Fisher Scientific alpha Amylase Polyclonal Antibody, HRP
Thermo Fisher Scientific의 Alpha Amylase Polyclonal Antibody, HRP 결합형은 Bacillus amyloliquefaciens 유래 단백질로 면역화된 Rabbit IgG 항체입니다. Western blot, ELISA, IP에 적합하며 고순도 이온교환 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens α-Amylase protein. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Alpha Amylase Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, and purified or partially purified α-Amylase (Bacillus amyloliquefaciens).
Cross-reactivity against α-Amylase from other tissues and species is unknown.
Target Information
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen.
The human genome contains several amylase genes expressed at high levels in salivary glands or pancreas. It is an amylase isoenzyme produced by the salivary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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