Thermo Fisher Scientific Alcohol Dehydrogenase Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:500-1:2,500

-

ELISA (ELISA)

1:4,000-1:20,000

-

Immunoprecipitation (IP)

1:1,000-1:5,000

-

Product Specifications

Species Reactivity

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Alcohol Dehydrogenase [Yeast]

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% gentamicin sulfate

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.

Target Information

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.