Thermo Fisher Scientific Human ULK1, GST Tag Recombinant Protein

Thermo Fisher Scientific Human ULK1, GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: -

Product Specifications

Product Specific Information

• For maximum recovery, spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use in original packaging until exhausted.
• For larger sizes, aliquoting is recommended to reduce freeze/thaw cycles (do not prepare aliquots below 20 µL).
• Never store kinase diluted.
• Properly stored at -80°C, guaranteed for 6 months from date of purchase.

Recombinant human ULK1 (1–649) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag.

Validated Applications:

• LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product: LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant
Gene Accession Number: NP_003556.1

Target Information

ULK1 is a serine/threonine protein kinase that plays a critical role in the initial stages of autophagy, a vital response to nutrient starvation. The conserved C-terminal domain (CTD) controls regulatory function and localization. Knockdown of ULK1 inhibits autophagic response and rapamycin-induced autophagy, consistent with its role downstream of mTOR.

ULK1 forms a complex with FIP200 and ATG13, essential for starvation-induced autophagy. These proteins are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.