Thermo Fisher Scientific EZH2 Monoclonal Antibody (OTI10B10), TrueMAB
EZH2 단백질을 인식하는 Mouse monoclonal antibody로, Western blot과 IHC(Paraffin)에서 검증됨. 인간 단백질에 반응하며, 무보존제 동결건조 형태로 제공. 재구성 및 결합 실험에 적합하며 연구용으로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 182-431 of human EZH2 (NP_004447) produced in E.coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
EZH2 (Histone-lysine N-methyltransferase) belongs to the Polycomb-group (PcG) family, which forms multimeric protein complexes involved in maintaining transcriptional repression across cell generations.
This protein interacts with embryonic ectoderm development protein, the VAV1 oncoprotein, and an X-linked nuclear protein.
EZH2 is implicated in hematopoietic and central nervous system regulation, and mutations in EZH2 can cause Weaver syndrome (WVS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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