Thermo Fisher Scientific Cytochrome C Monoclonal Antibody (6H2.B4)
Cytochrome C 단백질을 인식하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Native Cytochrome C에 특이적으로 반응하며, 단백질 A로 정제됨. 냉장 또는 -20°C에서 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 publications |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 5–20 µg/mL | 7 publications |
| ELISA | 0.1–1 µg/mL | - |
| Immunoprecipitation (IP) | 15 µg | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Rabbit, Rat |
| Published Species | Chicken, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H2.B4 |
| Immunogen | Rat Cytochrome C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | 2–8°C for up to 1 month; long-term at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533141 |
Available Conjugated Formats
- Alexa Fluor 488 (MA3-38200-A488)
- Alexa Fluor 555 (MA3-38200-A555)
- Alexa Fluor 647 (MA3-38200-A647)
- Request custom conjugation
Product Specific Information
This antibody reacts with native Cytochrome C, but does not recognize denatured Cytochrome C. It recognizes a region near amino acid 62 of rat Cytochrome C.
Target Information
Cytochrome C is an electron transport protein located in the mitochondrial intermembrane space, playing a key role in oxidative phosphorylation and ATP production. Upon apoptotic stimulation, Cytochrome C is released into the cytosol, where it binds apoptotic protease activating factor to initiate procaspase-9 activation. Cytosolic Cytochrome C subsequently activates caspase-3, a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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