Thermo Fisher Scientific GAL4 Monoclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:50 - 1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Yeast

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Immunogen

A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAL4,uniProtId:P04386-1,ncbiNodeId:-23,antigenRange:1-147,antigenLength:881,antigenImageFileName:33-8600_GAL4_P04386-1_House_mouse.svg,antigenImageFileNamePDP:33-8600_GAL4_P04386-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533143

Product Specific Information

33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.

Target Information

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.