Norgen Urine Exosome Purification Midi Kit
소변 시료(2~10 mL)에서 30분 이내에 순수하고 손상 없는 엑소좀을 정제할 수 있는 키트입니다. 초원심분리나 침전 시약 없이 사용 가능하며, 엑소좀 RNA 분석 및 NanoSight® 측정에 적합합니다. Norgen의 독자적 수지 기반 기술로 높은 순도와 회수율을 제공합니다.
- 브랜드
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- Exosome Reagents
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Norgen Urine Exosome Purification Midi Kit (Cat. 57800)
All-in-one system for the purification of urinary exosomes from different urine sample volumes.
Features and Benefits
- Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media, and saliva in less than 30 minutes
- Versatile sample input ranging from 250 µL to 30 mL
- Mini Kit: 250 µL – 1 mL urine
- Midi Kit: 2 mL – 10 mL urine
- Maxi Kit: 11 mL – 30 mL urine
- Based on Norgen’s proprietary resin separating matrix through exosome surface proteins
- No precipitation reagents, overnight incubation, protease, or coagulant treatments required
- No ultracentrifugation, filtration, or special syringes required
- Purifies intact exosomes (40–200 nm) depending on sample type
- Compatible with functional studies and NanoSight® or Electron Microscopy
- Exosomal RNA can be extracted using Norgen’s Exosomal RNA Purification technology or other RNA extraction methods
Product Overview
Norgen’s Urine Exosome Purification Kits provide a fast, reliable, and convenient method to purify and enrich intact exosomes and extracellular vesicles (EVs) from urine samples ranging from 250 µL to 30 mL.
The proprietary resin ensures high purity and avoids contamination with RNA-binding proteins.
NanoSight® Analysis
Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.
(이미지에는 밝은 점들이 흩어져 있는 입자 패턴이 나타나며, 엑소좀의 분포를 시각화합니다.)
Exosomal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit for downstream applications such as RT-qPCR, microarray, NGS, and biomarker discovery.
Supporting Data
Figure 1. Intact exosomes purified from 5 mL urine using different purification methods.
Norgen’s Midi Kit (Cat# 57800) shows recovery of 8.36 × 10⁹ particles/mL with exosome sizes from 75–250 nm and no impurities detected.
Figure 2. Comparison of exosomes purified using Norgen’s Midi Kit and Ultracentrifugation.
Norgen’s kit recovered 7.63 × 10⁸ particles/mL (65–195 nm), while ultracentrifugation recovered 1.56 × 10⁸ particles/mL (125–235 nm).
(그래프 정보: Concentration (×10⁸/mL) vs Particle Size (nm); 주요 피크 – Ultracentrifugation: 125, 165, 235 nm / Norgen: 95 nm)
Kit Specifications
| Specification | Details |
|---|---|
| Urine Input (Cat. 57700) | 250 µL – 1 mL |
| Urine Input (Cat. 57800) | 2 mL – 10 mL |
| Urine Input (Cat. 57900) | 11 mL – 30 mL |
| Size of Exosomes Purified | 40 – 150 nm |
| Elution Volume | Variable depending on urine input |
| Time to Complete 10 Purifications | 15 – 30 minutes |
Storage Conditions and Product Stability
All buffers should be tightly sealed and stored at room temperature.
Stable for 2 years after shipment.
Important Note
Urine samples stored at -70°C, -20°C, or 4°C may develop precipitates due to protein aggregation.
Removing these can cause exosome loss and affect nucleic acid quality.
Use Norgen’s Urine Preservative for stable storage at room temperature for over 2 years.
Kit Components
| Component | Cat. 57700 (50 preps) | Cat. 57800 (25 preps) | Cat. 57900 (15 preps) |
|---|---|---|---|
| Slurry E | 12.5 mL | 12.5 mL | 12.5 mL |
| ExoC Buffer | 8 mL | 30 mL | 50 mL |
| ExoR Buffer | 12 mL | 12 mL | 12 mL |
| Mini Filter Spin Columns (2 mL tubes) | 50 | 25 | 15 |
| Product Insert | 1 | 1 | 1 |
Documentation
Protocols
- Urine Exosome Purification Mini Kit (Cat. 57700)
- Urine Exosome Purification Midi Kit (Cat. 57800)
- Urine Exosome Purification Maxi Kit (Cat. 57900)
Safety Data Sheets
Flyers
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