Thermo Fisher Scientific CSK Recombinant Rabbit Monoclonal Antibody (1A2F5)
CSK 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ELISA에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 세포막 및 세포질에서 발현되는 CSK 단백질 검출에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1A2F5 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human CSK (P41240). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911759 |
Product Specific Information
- Positive test controls: A-549, Jurkat, Raji, C6, Mouse spleen, Mouse thymus, Rat lung, Rat spleen
- Target localization: Cell membrane, Cytoplasm
Immunogen sequence:
MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE
Target Information
CSK is a tyrosine kinase that downregulates the activity of the SRC oncoprotein through phosphorylation of its carboxy terminus. Since cell transformation by SRC is caused by mechanisms interfering with this phosphorylation, the CSK gene may function as an antioncogene.
CSK regulates cell growth, differentiation, migration, and immune responses. It phosphorylates tyrosine residues in the C-terminal tails of Src-family kinases including LCK, SRC, HCK, FYN, LYN, CSK, and YES1.
CSK is ubiquitously expressed as a ~50 kDa cytosolic protein, with a fraction localized to lipid rafts via interaction with PAG (Csk-binding protein).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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