Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human 및 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. 4°C 보관, 연구용 전용 제품.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue sections |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Zebrafish, Chicken, Sheep, Bovine, Rabbit, Guinea pig, Pig, Golden hamster, Cat, and Orangutan.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, existing as a tetramer of identical 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH plays roles in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It is implicated in apoptosis, neurodegenerative diseases, and cancer-related processes. GAPDH is also known to interact with amyloid precursor protein and Huntingtin protein.
Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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