Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI1G4), TrueMAB
L1CAM 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1G4). Western blot, ICC/IF, Flow cytometry에 적합. 인간 시료 반응성. Lyophilized 형태, PBS buffer(8% trehalose) 포함. 신경세포 발달 연구 및 CRASH 증후군 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G4 |
| Immunogen | Full length human recombinant protein of human L1CAM produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM (CD171) is an axonal glycoprotein of the immunoglobulin superfamily. Its ectodomain contains immunoglobulin-like and fibronectin type III domains, linked to a conserved cytoplasmic domain via a single transmembrane region.
This molecule is essential for nervous system development, including neuronal migration and differentiation. Mutations in L1CAM cause X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus).
Alternative splicing of a neuron-specific exon may have functional significance.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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