Thermo Fisher Scientific CD53 Monoclonal Antibody (7A8D3)
CD53 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 7A8D3). 다양한 응용 분야(WB, IHC, ICC/IF, Flow, ELISA)에 적합하며, Human 시료 반응성. 고순도 Protein G 정제, 안정적 액상 형태로 연구용에 최적화.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7A8D3 |
| Immunogen | Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091221 |
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, or tetraspanins CD37, CD81, and CD82, modulating various signaling processes. CD53 is involved in radioresistance of tumor cells and has an anti-apoptotic effect upon triggering. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development and strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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