Thermo Fisher Scientific CD53 Monoclonal Antibody (1H2C7)
CD53 단백질을 인식하는 mouse IgG1 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용 가능. 인간 시료 반응성, Protein G 정제, 액상 형태로 공급되며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H2C7 |
| Immunogen | Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090991 |
Target Information
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, and other tetraspanins (CD37, CD81, CD82), modulating various signaling processes. CD53 contributes to tumor cell radioresistance and exhibits anti-apoptotic effects upon activation. In thymus, CD53 expression increases during T cell positive selection and is strongly induced in macrophages exposed to bacterial lipopolysaccharide, while neutrophil stimulation leads to down-regulation of CD53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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