Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11)
Thermo Fisher Scientific의 CD68 Monoclonal Antibody (FA-11)은 마우스 CD68(macrosialin)을 인식하는 Rat IgG2a 항체로, 다양한 면역분석(WB, IHC, Flow Cytometry 등)에 사용됩니다. 단백질 G 정제, 액상 형태, 1 mg/mL 농도이며 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 10 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | - | 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FA-11 |
| Immunogen | Purified Con A acceptor glycoprotein from P815 cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C or -20°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538168 |
Product Specific Information
- Non-reducing conditions are recommended for Western blot applications.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
- Rat anti-Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68.
- CD68 is classified as a unique scavenger receptor (ScR) family member due to the presence of a lysosome-associated membrane protein (LAMP)-like domain.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages, and to a lesser extent by dendritic cells and peripheral blood granulocytes.
It may play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular interactions.
CD68 is expressed by interdigitating reticulum cells in tonsil and certain histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.
Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including AML.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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