Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow 등 다양한 분석에 적합. Tight junction 단백질 연구 및 세포 간 결합 신호전달 연구에 활용 가능. 고순도 정제 및 안정한 액상 형태로 제공.
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Thermo Fisher Scientific ZO-1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | View publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1:10–1:50 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide between 1570–1600 amino acids from the C-terminal region of human ZO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion, Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2542216 |
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins also associate with claudin, occludin, and F-actin at tight junction strands, providing linkage between the actin cytoskeleton and the tight junction.
ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, while ZO-1 is also expressed at cell junctions of cardiac myocytes.
ZO-1 may play a role in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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