Thermo Fisher Scientific Thymidylate Synthase Polyclonal Antibody
Thymidylate Synthase 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, IP 등에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 시약으로 DNA 합성 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 35–313 of Human Thymidylate Synthase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545343 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, THP-1, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2
- Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (86%), Xenopus laevis (85%), Dog (94%), Pig (95%), Rhesus Monkey (98%), Chimpanzee (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis.
TS is a critical target for fluoropyrimidines, an important group of antineoplastic drugs used in the treatment of solid tumors.
Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP, which inactivates TS by forming a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate.
Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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