Thermo Fisher Scientific TFAM Polyclonal Antibody
TFAM 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF, IP에 적합합니다. 인간, 마우스, 랫트에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 미토콘드리아 DNA 복제 및 전사 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–246 of Human mtTFA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.23 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545341 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Predicted reactivity: Chimpanzee (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial transcription factor A (TFAM) is a nuclear-encoded protein that translocates to mitochondria, where it plays a crucial role in maintaining and expressing mitochondrial DNA (mtDNA). TFAM regulates mtDNA replication and transcription, ensuring proper mitochondrial function. It contains an LC3 interacting region (LIR) motif, enabling binding to autophagy proteins such as LC3, which supports autolysosomal degradation of leaked mtDNA and limits inflammation.
TFAM levels vary across tissues, influencing mitochondrial function. High TFAM levels can repress mtDNA expression and affect oxidative phosphorylation (OXPHOS) in some tissues, while in others, such as the heart, increased mtDNA copy number maintains balanced TFAM-to-mtDNA ratios. Disruptions in TFAM are associated with mitochondrial dysfunction, neurodegeneration, and other mtDNA-related diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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