Thermo Fisher Scientific alpha Actinin 2 Polyclonal Antibody
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다양한 종(Human, Mouse, Rat 등)에 반응하는 alpha Actinin 2 폴리클로날 항체. WB, IHC, ICC 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–377 of Human alpha Actinin 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545339 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, Rat heart
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha actinins belong to the spectrin gene superfamily, representing a diverse group of cytoskeletal proteins including alpha and beta spectrins and dystrophins.
Alpha actinin is an actin-binding protein with multiple roles in different cell types.
- In nonmuscle cells: Found along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells (skeletal, cardiac, smooth): Localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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