Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 표지된 염소 유래 항-마우스 IgG (H+L) 2차 항체로, 면역세포염색(ICC/IF)에 최적화되어 있습니다. F(ab')2 형태로 비특이적 결합을 최소화하며, 교차흡착 처리로 다양한 종 단백질과의 반응을 억제합니다. 형광 검출용으로 높은 신뢰성과 재현성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535998 |
| Target | IgG |
| Cross Adsorption | Against bovine, horse, human, pig and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product has been overfilled to ensure complete recovery.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Shelf life: 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, rabbit, or swine.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse-derived targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondaries can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
A variety of conjugate formats are available for this antibody, including:
- Agarose, Alexa Fluor series (350–790), Alexa Fluor Plus series, AP, APC, Biotin, BODIPY FL, Cyanine3/5, DyLight series, FITC, HRP, Pacific Blue/Orange, PE, Rhodamine, Texas Red-X, and more.
- Custom conjugation available upon request.
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