Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC 결합된 F(ab')2 형태의 Goat anti-Mouse IgG (H+L) 항체로, 높은 교차흡착을 통해 비특이적 결합 최소화. 면역세포 형광염색(IC/IF)에 최적화되어 있으며, 552/578 nm에서 발광. 연구용으로 높은 순도와 안정성 제공.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 0.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation/Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles). Keep in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535992 |
| Target | IgG |
| Cross Adsorption | Bovine, goat, human, rabbit, and rat IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand for 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and discard after use.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, bovine, goat, human, rabbit, or rat IgG.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of mouse targets.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
Available Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- AP, Biotin, DSB-X Biotin, FITC, HRP
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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