
Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 F(ab`)2 형태의 Goat anti-Mouse IgG (H+L) 교차 흡착 2차 항체로 높은 특이성과 민감도를 제공. Western blot, IHC, ELISA 등 다양한 면역 분석에 적합. 비특이적 결합 최소화 및 안정적 저장 가능.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535993 |
| Target | IgG |
| Cross Adsorption | Against bovine, horse, human, pig and rabbit serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, rabbit, or swine.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of mouse targets.
Secondary antibodies provide versatility for multiple detection systems (HRP, AP, fluorescence) and offer enhanced sensitivity through signal amplification.
Typically produced by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Available Conjugate Formats
- Agarose
- Alexa Fluor series (350–790, Plus variants)
- AP, APC, APC-Alexa Fluor 750
- Biotin, Biotin-XX
- BODIPY FL
- Cyanine3, Cyanine5
- DyLight series (350–800)
- FITC
- Pacific Blue, Pacific Orange
- PE, PE-Alexa Fluor 610
- Rhodamine, Rhodamine Red-X
- Texas Red-X, TRITC
- Custom conjugation available upon request
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