Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), PerCP
HSF1 단백질을 검출하기 위한 PerCP 결합 Rat 모노클로날 항체로, Western blot, IHC, ICC, ELISA 등에 사용 가능. 다양한 종에 반응하며 고순도 Protein G 정제. 열충격 스트레스 연구에 적합한 고감도 항체.
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Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H8 |
| Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378–395 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931583 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45129 detects HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot using Goat anti-rat IgG: HRP as secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
Heat shock factor 1 (HSF1) regulates transcription of heat shock genes by binding to heat shock response elements (HSEs), composed of three repeats of a 5-nucleotide {nGAAn} module.
HSF1 and HSF2 share 38% sequence identity and respond to distinct stimuli.
HSF1 is activated by heat, heavy metals, and oxidative stress, transitioning from a monomeric to trimeric nuclear state that binds DNA.
Phosphorylation of HSF1 is essential for maximal transcriptional activation of heat shock genes.
Usage
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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