Thermo Fisher Scientific HSF2 Monoclonal Antibody (3E2), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

Immunocytochemistry (ICC/IF)

1:200

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E2

Immunogen

Purified recombinant mouse HSF2 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSF2,uniProtId:P38533-1,ncbiNodeId:10090,antigenRange:1-535,antigenLength:535,antigenImageFileName:MA5-45133_HSF2_P38533-1_Rat.svg,antigenImageFileNamePDP:MA5-45133_HSF2_P38533-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

4 µg/mL of MA5-45133 was sufficient for detection of HSF2 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.|Detects approximately 69kDa.

Target Information

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.