Thermo Fisher Scientific CD40 Monoclonal Antibody (UMAB183), UltraMAB

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:200

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The CD40 antigen is a single-chain glycoprotein belonging to the tumor necrosis factor/nerve growth factor superfamily. It shows significant homology to the Hodgkin’s disease-associated antigen CD30. CD40 is expressed on all B cells except plasma cells and is also found on some epithelial cells, carcinomas, and lymphoid dendritic cells.

CD40 plays a key role in immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

The transcription factor AKNA regulates CD40 expression, influencing cell interactions. CD40 interacts with adaptor protein TNFR2, mediating signal transduction. The CD40–CD40L interaction is implicated in amyloid-beta-induced microglial activation, suggesting involvement in early Alzheimer’s disease pathogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

Diseases associated with CD40 dysfunction include:

• Type 3 Hyper-IgM Immunodeficiency
• CD40 Ligand Deficiency

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.